Periplasmic fraction of total cell volume in E. coli and Salmonella typhimurium under different growth conditions

Range Table - link
Organism Various
Reference Stock JB, Rauch B, Roseman S. Periplasmic space in Salmonella typhimurium and Escherichia coli. J Biol Chem. 1977 Nov 10 252(21):7850-61 p.7858 table IVPubMed ID334768
Method P.7851 left column 3rd paragraph: "The cytoplasmic, periplasmic, and total cell volumes were all derived from the distributions of solutes in cell suspensions, The rationale is illustrated in Fig. 1. Inulin, because it is large, cannot penetrate the outer membrane and is located only in the extracellular fluid. Sucrose, without the aid of a permease, cannot cross the plasma membrane and is located only in the periplasm and extracellular fluid. Water moves freely throughout the cell, it is located in the cytoplasm, periplasm, and extracellular fluid."
Comments P.7853 right column 5th paragraph: "For instance, results obtained with S. typhimurium grown and suspended in nutrient broth were similar to those obtained with cells grown, washed, and suspended in Medium 63 (see Table IV). Several different strains of enteric bacteria were examined to determine whether the compartmental structure observed in S. typhimurium LT2 was a general feature of these types of organisms. As shown in Table IV, a similar division of cellular contents into periplasm and cytoplasm occurs in lipopolysaccharide-defective strains of S. typhimurium (SL3555 and SL756) and in different strains of E. coli (K12 and ML308-255)."
Entered by Uri M
ID 104053