Amount of protein in S. typhimurium LT2 at different growth rates

Range 99 and 521 for doubling time of 2.24hours and 17.3min respectively table link fg/cell
Organism Bacteria Salmonella typhimurium
Reference Aldea M, Herrero E, Esteve MI, Guerrero R. Surface density of major outer membrane proteins in Salmonella typhimurium in different growth conditions. J Gen Microbiol. 1980 Oct120(2):355-67. p.360 table 2PubMed ID6262433
Method P.357 3rd paragraph: "Protein concentration was calculated by the Lowry method, using a 50 mM-Tris/HC1 pH 6.8 solution as standard." See note above table. For conditions of growth see note above table 1 - link
Comments P.359 bottom paragraph: "As indicated in Table 2, the proportion of major OMP [outer membrane protein] with respect to total protein was lower in rich media than in poorer media. However, the density of the four major OMP per unit of surface area did not significantly change in the different growth conditions (Fig. 1 b and Table 2). When the porins and the 33K protein were analysed separately, the ratio of one to the other did not change over the whole range of growth rates tested, porins representing about 45% of the major OMP (Table 2)." 99fg at doubling time of 2.24hours. 521fg at doubling time of 17.3minutes.
Entered by Uri M
ID 105868