| Comments |
P.7853 right column 2nd paragraph: "Results such as those presented in Fig. 3 indicated that, after correcting for material contained in the cytoplasm, the intracellular level of any low molecular weight neutral solute could be used to determine periplasmic volume. This conclusion was substantiated by measuring the distributions of several small solutes in addition to TMG [methyl β-D-thiogalactopyranoside] and sucrose. As shown in Table III, the same periplasmic volumes were obtained with mannitol, N-acetylglucosamine, sucrose, and TMG. In this experiment, a strain of S. typhimurium defective in its ability to transport sugars was used so as to minimize cytoplasmic uptake." See note above table |