| Range |
>500 mRNAs/hour
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. p.339 left column bottom paragraphPubMed ID21593866
|
| Method |
P.337 right column top paragraph: "Newly synthesized RNA can be labelled with the nucleoside analogue 4-thiouridine (4sU).
4sU-containing mRNA can be purified and compared with the preexisting fraction to compute mRNA half-lives [ref 10]." See model in p.339 fig. 3a |
| Comments |
P.339 left column bottom paragraph: "Average cellular transcription rates predicted by the model spanned two orders of magnitude with a median of about two mRNA molecules per hour [Fig. 3b]. An extreme example was Mdm2 with more than 500 mRNAs per hour." NIH3T3 (mouse fibroblast) cells. |
| Entered by |
Uri M |
| ID |
106380 |