Average cellular transcription rates predicted by model
| Value | 2 mRNA/hour |
|---|---|
| Organism | Mammalian tissue culture cell |
| Reference | Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. p.339 left column 3rd paragraph and fig.3bPubMed ID21593866 |
| Method | Newly synthesized RNA can be labelled with the nucleoside analogue 4-thiouridine (4sU). 4sU-containing mRNA can be purified and compared with the preexisting fraction to compute mRNA half-lives. See model in p.339 fig. 3a |
| Comments | NIH3T3 (mouse fibroblast) cells. Average cellular transcription rates predicted by the model spanned two orders of magnitude with a median of about two mRNA molecules per hour. |
| Entered by | Uri M |
| ID | 106379 |