| Range |
0.004-0.006 µm^2/sec
|
| Organism |
Human Homo sapiens |
| Reference |
Mor A, Suliman S, Ben-Yishay R, Yunger S, Brody Y, Shav-Tal Y. Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells. Nat Cell Biol. 2010 Jun12(6):543-52 p. 547 fig 3e and p. 546 table 1, Table - link PubMed ID20453848
|
| Method |
To characterize in vivo export kinetics at the single mRNP (mRNA-protein complex) level, and
to understand the relative temporal contribution of export to the total
kinetics of nucleocytoplasmic translocation, researchers established a mammalian
cell system harbouring stably integrated genes that enabled the
tracking of single mRNPs from the time of transcription and during
nuclear transport and export. Gene constructs containing different forms of the human dystrophin
(Dys) cDNA fused to a fluorescent protein (green fluorescent protein
(GFP) or Cerulean (Cer)) were generated. Active transcription sites and single mRNPs were detected by transient
transfection with YFP–MS2-NLS (nuclear localization sequence). |
| Comments |
Human U2OS (osteosarcoma)
cells. A wide range of Dys-mRNP nucleoplasmic diffusion
rates were measured, ranging on an average D of 0.004–0.006 µm^2/s |
| Entered by |
Uri M |
| ID |
105651 |