Diffusion coefficient of polyA mRNA in nucleoplasm of U2OS (human osteosarcoma) cells
| Value | 0.04 µm^2/sec |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Molenaar C, Abdulle A, Gena A, Tanke HJ, Dirks RW. Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells. J Cell Biol. 2004 Apr 26 165(2):191-202. Table - link PubMed ID15117966 |
| Method | Poly(A)+ RNA was visualized in nuclei of living U2OS (human osteosarcoma) cells by means of a tetramethylrhodamine (TAMRA)-labeled 2'OMe (U)22 probe that is complementary to the poly(A) tail of mRNAs. Kinetics measured by FLIP (Fluorescence Loss in Photobleaching) and FRAP (Fluorescence recovery after photobleaching) |
| Comments | See BNID 105292 |
| Entered by | Uri M |
| ID | 105294 |