| Value |
1.8e-4
correct/incorrect nucleotide
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Alic N, Ayoub N, Landrieux E, Favry E, Baudouin-Cornu P, Riva M, Carles C. Selectivity and proofreading both contribute significantly to the fidelity of RNA polymerase III transcription. Proc Natl Acad Sci U S A. 2007 Jun 19 104(25):10400-5 abstractPubMed ID17553959
|
| Method |
P.10400 right column bottom paragraph: "In this study, [investigators] examined nucleotide misincorporation by cleavage-competent and cleavage-deficient S. cerevisiae Pol III ternary complexes at a specific position on a tRNA gene. The results define the strategies used by a eukaryotic RNA Pol with high intrinsic cleavage activity to ensure the accuracy of its transcription. [They] used computational methods to combine the experimental observations and predict the steady-state error rate of Pol III and to demonstrate the equally high contribution of proofreading and nucleotide selectivity to the overall fidelity of Pol III." |
| Comments |
Abstract: "Determination of relative rates of the reactions producing correct and erroneous transcripts at a specific position on a tRNA gene, combined with computational methods, demonstrated that Pol III has a highly efficient proofreading activity increasing its transcriptional fidelity by a factor of 10^3 over the error rate determined solely by selectivity (1.8×10^-4)." See BNID 103453, 105213 |
| Entered by |
Uri M |
| ID |
105213 |