Kcat (turnover rate) of activation of leucine by Leu tRNA Synthetase

Value 11 Sec^-1 Range: ±0.4 Sec^-1
Organism Bacteria Escherichia coli
Reference Lue SW, Kelley SO. An aminoacyl-tRNA synthetase with a defunct editing site. Biochemistry. 2005 Mar 1 44(8):3010-6 Table - link PubMed ID15723544
Method Cloning and Preparation of tRNA Constructs, Preparation of hs mt, WT E. coli, and E. coli T252Y LeuRS, Isolation of hs mt tRNALeu and E. coli tRNALeu Misacylated with Isoleucine, Deacylation of hs mt Ile-tRNALeu and E. coli Ile-tRNALeu, Cross-Aminoacylation and Misaminoacylation Assays,
Comments Kinetic parameters were determined at pH 7.5 and 37 °C. For Kcat of human mitochondrial LeuRS see BNID 104978
Entered by Uri M
ID 104977