Kinetic Parameters for the Activation of Leucine and Isoleucine by E. coli and human mitochondrial Leu tRNA Synthetase

Range Table - link
Organism Various
Reference Lue SW, Kelley SO. An aminoacyl-tRNA synthetase with a defunct editing site. Biochemistry. 2005 Mar 1 44(8):3010-6PubMed ID15723544
Method Cloning and Preparation of tRNA Constructs, Preparation of hs mt, WT E. coli, and E. coli T252Y LeuRS, Isolation of hs mt tRNALeu and E. coli tRNALeu Misacylated with Isoleucine, Deacylation of hs mt Ile-tRNALeu and E. coli Ile-tRNALeu, Cross-Aminoacylation and Misaminoacylation Assays,
Comments Kinetic parameters were determined at pH 7.5 and 37 °C.
Entered by Uri M
ID 104976