Kcat (turnover rate) of activation of leucine by Leu tRNA Synthetase
| Value | 11 Sec^-1 Range: ±0.4 Sec^-1 |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Lue SW, Kelley SO. An aminoacyl-tRNA synthetase with a defunct editing site. Biochemistry. 2005 Mar 1 44(8):3010-6 Table - link PubMed ID15723544 |
| Method | Cloning and Preparation of tRNA Constructs, Preparation of hs mt, WT E. coli, and E. coli T252Y LeuRS, Isolation of hs mt tRNALeu and E. coli tRNALeu Misacylated with Isoleucine, Deacylation of hs mt Ile-tRNALeu and E. coli Ile-tRNALeu, Cross-Aminoacylation and Misaminoacylation Assays, |
| Comments | Kinetic parameters were determined at pH 7.5 and 37 °C. For Kcat of human mitochondrial LeuRS see BNID 104978 |
| Entered by | Uri M |
| ID | 104977 |