| Primary Source |
S. Bauer, J. Shiloach, Maximal exponential growth rate and yield of E. coli obtainable in a bench-scale fermentor, Biotechnol Bioeng, 16 (1974), pp. 933–941 DOI: 10.1002/bit.260160707 AND J. Shiloach, S. Bauer, High yield growth of E. coli at different temperatures in a bench scale fermentor, Biotechnol Bioeng, 17 (1975), pp. 227–239 DOI: 10.1002/bit.260170208 link AND S. Bauer, E. Ziv, Dense growth of aerobic bacteria in a bench-scale fermentor, Biotechnol Bioeng, 18 (1976), pp. 81–94 DOI: 10.1002/bit.260180107 AND K. Nakano, M. Rischke, S. Sato, H. Märkl, Influence of acetic acid on the growth of Escherichia coli K12 during high-cell-density cultivation in a dialysis reactor, Appl Microbiol Biotechnol, 48 (1997), pp. 597–601 PubMed ID4606791, 813791, 9421923
|
| Comments |
P.354 2nd column: "The key physical element in high-density fermentation systems is adequate oxygen supply to the growing culture, which can be achieved only by supplementing or substituting the air supply with pure oxygen. But even with pure oxygen, it is possible to obtain high-density E. coli growth only if either temperature, availability of other nutrients, or both limit the growth rate. Exponential growth of high-density E. coli culture cannot exceed 50 g/l dcw (primary sources Bauer and Shiloach, 1974, Shiloach and Bauer, 1975 and Bauer and Ziv, 1976) on the other hand, slow growing cultures can grow to densities as high as 190 g/l dcw, provided key metabolic obstacles are resolved (primary source Nakano et al., 1997)." Note-units seem to be "g dcw/L" rather than "g/l dcw". See BNID 104943. Counter-intuitively, as nutrient levels in medium increase they become inhibitory and a lower cell density can be reached ~15g/L for the maximum non-inhibitive concentration of nutrients (BNID 105323). |