Maximum optical density and doubling time of E. coli BL21 (DE3) aerobic batch cultures in different minimal media supplemented with 0.4% glycerol

Range Table - link
Organism Bacteria Escherichia coli
Reference Gunasekera TS, Paliy O. Growth of E. coli BL21 in minimal media with different gluconeogenic carbon sources and salt contents. Appl Microbiol Biotechnol. 2007 Jan73(5):1169-72. p.1171 Table 1PubMed ID16944129
Method P.1170 right column top paragraph: "Growth was monitored spectrophotometrically by measuring each culture’s OD600nm periodically." P.1171 left column bottom paragraph: "[Investigators] also examined the aerobic growth of BL21 in different minimal media. Three different minimal media were used: M9 minimal medium, commonly used in microbiological studies N−C− minimal medium, utilized for physiological experiments and gene expression studies (Soupene et al. 2003 Gyaneshwar et al. 2005) and LR medium, used for preparation of deuterated components in E. coli (LeMaster and Richards 1982 Paliy et al. 2003)."
Comments P.1171 left column bottom paragraph: "Compared to N−C− and M9 media, LR medium allowed BL21 cultures to achieve greater final cell density and to maintain substantially better growth rates in the exponential phase of growth (Table 1). The results were independent of the source of water (deionized vs distilled) used for medium preparation. Similar differences between M9 and LR media were observed for the growth of NCM3722 strain (data not shown). When medium recipes were compared, the observed growth differences were thought to be attributed to the presence of bivalent iron and to the higher phosphate content of the LR medium. When M9 medium was supplemented with ferrous sulphate or additional potassium phosphate (FeSO4·7H2O and KH2PO4 were added, respectively) so as to achieve the concentrations present in the LR medium, BL21 strain grew well, with a growth rate and final OD600 values substantially better than those for the original M9 medium (Table 1)."
Entered by Uri M
ID 105394