| Value |
20.5
g/L
Range: Table - link g/L
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Puskeiler R, Kaufmann K, Weuster-Botz D. Development, parallelization, and automation of a gas-inducing milliliter-scale bioreactor for high-throughput bioprocess design (HTBD). Biotechnol Bioeng. 2005 Mar 5 89(5):512-23 DOI: 10.1002/bit.20352 abstract & p.519 table IPubMed ID15669089
|
| Primary Source |
See refs beneath table |
| Method |
Abstract: "A novel milliliter-scale bioreactor equipped with a gas-inducing impeller was developed with oxygen transfer coefficients as high as in laboratory and industrial stirred-tank bioreactors. The bioreactor reaches oxygen transfer coefficients of >0.4 s^-1. Oxygen transfer coefficients of >0.2 s^-1 can be maintained over a range of 8- to 12-mL reaction volume. A reaction block with integrated heat exchangers was developed for 48-mL-scale bioreactors. The block can be closed with a single gas cover spreading sterile process gas from a central inlet into the headspace of all bioreactors. The gas cover simultaneously acts as a sterile barrier, making the reaction block a stand-alone device that represents an alternative to 48 parallel-operated shake flasks on a much smaller footprint. Process control software was developed to control a liquid-handling system for automated sampling, titration of pH, substrate feeding, and a microtiter plate reader for automated atline pH and atline optical density analytics. The liquid-handling parameters for titration agent, feeding solution, and cell samples were optimized to increase data quality. A simple proportional pH-control algorithm and intermittent titration of pH enabled Escherichia coli growth to a dry cell weight of 20.5 g/L in fed-batch cultivation with air aeration." |
| Comments |
P.518 left column 2nd paragraph: "The application of a liquid handler proved useful to control a pH-controlled fed-batch cultivation of E. coli with a reaction volume of 5 mL. Reaching 20 g/L DCW demonstrated the high oxygen transfer capacity of the developed milliliter-scale bioreactor. Table I summarizes recently published results of cultivations in miniature bioreactors designed for parallel operation." See BNID 104943 for maximal densities in other techniques and growth media |
| Entered by |
Uri M |
| ID |
104941 |