Cell concentration for Luria Bertani culture with OD600 of 0.1

Value 2e+7 cells/ml
Organism Bacteria Escherichia coli
Reference Sezonov G, Joseleau-Petit D, D'Ari R. Escherichia coli physiology in Luria-Bertani broth. J Bacteriol. 2007 Dec189(23):8746-9 p.8747 fig. 1aPubMed ID17905994
Method P.8746 right column 3rd paragraph: "The widely used wild-type E. coli K-12 strain MG1655 (ref 1), which has been sequenced and annotated (refs 2, 10), was chosen for this study. An overnight culture in Luria-Bertani broth was diluted 5,000-fold in 250 ml fresh medium in a 1-liter Erlenmeyer flask and cultivated in a shaking water bath (180 rpm) at 37°C. The OD600 and number of cells per milliliter were monitored." Measured visually from fig. 1A
Comments Note that cell concentration of a different strain with OD600 of 0.1 is 10^8 cells/ml, greater by a factor of 5 (see BNID 100985). The optical density of yeast and bacterial cultures is due to light scattering, not absorption. Therefore it is sensitive to the geometry of the detector relative to the cuvette, as well as the size and shape of the cells. You really have to calibrate each spectrophotometer for each strain. See BNID 104831
Entered by Uri M
ID 103625