| Value |
79000
picomole/mg protein
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Ganong BR, Raetz CR. Massive accumulation of phosphatidic acid in conditionally lethal CDP-diglyceride synthetase mutants and cytidine auxotrophs of Escherichia coli. J Biol Chem. 1982 Jan 10 257(1):389-94 Table - link PubMed ID6273438
|
| Method |
When 100 micrograms/ml erythromycin is added to nutrient agar plates, it is possible to obtain a 30-fold enrichment for cds mutants from a mutagen-treated stock, as judged by colony autoradiography. Using this approach researchers isolated 38 new cds mutants, nine of which are unable to grow at a culture pH greater than 8. Protein levels were calculated from the A550 of the culture |
| Comments |
In GN85 (cds+) at pH 6, the liponucleotides comprised about 0.04% of total phospholipids, or about a fifth of the size of the phosphatidic acid pool (See Table - link See BNID 104812, 104814 |
| Entered by |
Uri M |
| ID |
104813 |