Liponucleotide levels in 2 strains at pH6 and 8.5

Range Table - link
Organism Bacteria Escherichia coli
Reference Ganong BR, Raetz CR. Massive accumulation of phosphatidic acid in conditionally lethal CDP-diglyceride synthetase mutants and cytidine auxotrophs of Escherichia coli. J Biol Chem. 1982 Jan 10 257(1):389-94PubMed ID6273438
Method When 100 micrograms/ml erythromycin is added to nutrient agar plates, it is possible to obtain a 30-fold enrichment for cds mutants from a mutagen-treated stock, as judged by colony autoradiography. Using this approach researchers isolated 38 new cds mutants, nine of which are unable to grow at a culture pH greater than 8
Comments In GN85 (cds+) at pH 6, the liponucleotides comprised about 0.04% of total phospholipids, or about a fifth of the size of the phosphatidic acid pool (See Table - link)
Entered by Uri M
ID 104812