Levels of thioredoxins and glutaredoxins in wild type (CSH7) strain

Range Grx1 ~1,000: Grx2 ~10,000: Grx3 ~9,000: Trx1 ~4,250: Trx2 ~300 ng/mg total protein
Organism Bacteria Escherichia coli
Reference Potamitou A, Holmgren A, Vlamis-Gardikas A. Protein levels of Escherichia coli thioredoxins and glutaredoxins and their relation to null mutants, growth phase, and function. J Biol Chem. 2002 May 24 277(21):18561-7. DOI: 10.1074/jbc.M201225200 link p.18564 figure 1B PubMed ID11893749
Method Abstract: "Levels of Escherichia coli thioredoxin 1 (Trx1), Trx2, glutaredoxin 1 (Grx1), Grx2, and Grx3 have been determined by novel sensitive sandwich enzyme-linked immunosorbent assay." Values extracted visually from figure 1B
Comments P.18563 right column bottom paragraph: "Thioredoxin and Glutaredoxin Levels in Catalase (katEkatG) Minus Strains—In katEkatG minus strains, the levels of Trx1 and Trx2 did not change significantly compared with those of Grx1, Grx2, and Grx3, which increased 2-fold. The effect in the levels of glutaredoxins was even more pronounced in strains lacking parts of the glutaredoxin or thioredoxin system (gor-, gshA-, and trxB-) (Fig. 1), with Grx2 reaching >20 µg/mg in the katEkatGgshA minus strain. These data correlate well with the reported up-regulation of total glutaredoxin activity in the same strains (ref 8)."
Entered by Uri M
ID 113254