Percent of phosphatidylethanolamine out of total Phospholipid composition at pH 8.5

Value 74.5 %
Organism Bacteria Escherichia coli
Reference Ganong BR, Raetz CR. Massive accumulation of phosphatidic acid in conditionally lethal CDP-diglyceride synthetase mutants and cytidine auxotrophs of Escherichia coli. J Biol Chem. 1982 Jan 10 257(1):389-94 Table - link PubMed ID6273438
Method When 100 micrograms/ml erythromycin is added to nutrient agar plates, it is possible to obtain a 30-fold enrichment for cds mutants from a mutagen-treated stock, as judged by colony autoradiography. Using this approach researchers isolated 38 new cds mutants, nine of which are unable to grow at a culture pH greater than 8
Comments At pH 6 in GN85 (cds+) strain. phosphatidylglycerol + cardiolipin constituted 25.3% of phospholipids and phosphatidic acid constituted 0.2% of total phospholipids. See BNID 104809, 104810
Entered by Uri M
ID 104811