Rates of spontaneous mutation in DNA-based microbes

Range Table - link
Organism Various
Reference Drake JW, A constant rate of spontaneous mutation in DNA-based microbes.Proc Natl Acad Sci U S A. 1991 Aug 15 88(16):7160-4 p.7163 Table 1PubMed ID1831267
Method Phage M13-"The mutational target is 258bp of an inserted E. coli lacZa sequence [ref 5]." Phage T2-"The mutational target is the rII locus, whose size is taken to be the same as in the closely related phage T4 (see below), namely 3136 bp." phage T4-"The mutational target is the 3136bp of the rII locus [refs 18-21]."
Comments "The average mutation rate per base pair is inversely proportional to genome size. Therefore, a nearly invariant microbial mutation rate appears to have evolved." "The mutation rates in Table 1 were obtained by using cells growing in an often rich mixture of nutrients in a usually aerobic environment. Thus, the mutations will have arisen as a simple mixture of unforced errors of DNA replication plus the consequences of DNA damage directly attendant upon ordinary cellular metabolism and tightly linked temporally with DNA replication itself." For a value of 0.0001-0.0002 substitutions/generation, see BNID 105029, 105031
Entered by Uri M
ID 103918