Mutation rate per base pair per replication (very old estimate, see below)

Value 5.4E-10 mutation/bp/replication Range: Table - link mutation/bp/replication
Organism Bacteria Escherichia coli
Reference Drake JW, A constant rate of spontaneous mutation in DNA-based microbes.Proc Natl Acad Sci U S A. 1991 Aug 15 88(16):7160-4 p.7163 table 1PubMed ID1831267
Method Value is mean of two genes-LacI (two measurements) and hisGDCBHAFE (one measurement). P.7160 right column 3rd paragraph: "Two methods are commonly used to calculate mutation rates in microbes. One is the fluctuation test of Luria and Delbruck (ref 2). Many replicate cultures are grown from small mutant-free inocula until roughly half have experienced a mutation, and the cultures are then scored for average population size and for mutants. The mutation rate can then be calculated either by using the entire distribution (method F) or by using only the fraction of cultures without mutants (method FO). Another method is that of mutant accumulation (3). In one protocol (method A), a population is grown until large enough to contain many mutants and the mutant frequency is then followed as a function of population size. Then µ=(f2-f1)/ln(N2/N1), where µ=mutation rate per replication, f=mutant frequency (at time 1 or 2) and N=population size (at time 1 or 2)."
Comments For mutation rate per base pair per replication and mutation rate per base pair per generation in human See BNID 105095, 100414, respectively
Entered by Ron Milo - Admin
ID 100263