| Method |
Abstract: "HeLa cells in monolayer cultures were treated with the following inhibitors of DNA synthesis: mitomycin C, nitrogen mustard, fluorodeoxyuridine, hydroxyurea, arabinofuranosylcytosine and high concentrations of thymidine. The concentration of each inhibitor used was, in most cases, just sufficient to arrest cell multiplication and all produced unbalanced growth in the sense that the synthesis of RNA and protein were only partially inhibited while DNA synthesis stopped." P.332 right column top paragraph: "Three days were allowed
for the cells to adhere to the Petri dishes and to enter the logarithmic phase of growth." P.332 right column bottom paragraph: "Cell number was obtained with a Coulter counter, while cell sizing was performed with the automatic particle size distribution analyser Model J Electronic Co., Hialeah, Fla." |