catalytic rate of rhodopsin in vitro
| Value | 46 sec^-1 |
|---|---|
| Organism | Generic |
| Reference | Ernst OP, Gramse V, Kolbe M, Hofmann KP, Heck M. Monomeric G protein-coupled receptor rhodopsin in solution activates its G protein transducin at the diffusion limit. Proc Natl Acad Sci U S A. 2007 Jun 26104(26):10859-64. fig 5PubMed ID17578920 |
| Method | Construction of R–mVenus, R–mECFP, and mVenus–mECFP Fusion Plasmids, Rhodopsin, R–mVenus, R–mECFP, and mVenus–mECFP fusion proteins were expressed in COS-1 cells by using a diethylamino-ethyl (DEAE)-dextran transfection procedure, purification of rhodopsin, Preparation of Bovine Rhodopsin and SEC. |
| Comments | In the native membrane catalytic rates are an order of magnitude higher. This may be explained by the reduction in translational degrees of freedom in a two-dimensional membrane, which facilitates successful coupling |
| Entered by | Uri M |
| ID | 103609 |