Rate of first step in catalytic cycle of Amplex red by Horseradish peroxidase

Value 9e+6 M^-1×sec^-1
Organism Generic
Reference Gorris HH, Walt DR. Mechanistic aspects of horseradish peroxidase elucidated through single-molecule studies. J Am Chem Soc. 2009 May 6, 131(17):6277-82PubMed ID19338338
Primary Source Chance B. The kinetics and stoichiometry of the transition from the primary to the secondary peroxidase peroxide complexes. Arch Biochem Biophys. 1952 Dec41(2):416-24.PubMed ID13008459
Method Enclosing a population of hundreds of single enzyme molecules in a large array of femtoliter reaction chambers and observing the individual molecules by fluorescence microscopy
Comments Hydrogen peroxide (H2O2) initiates the peroxidase catalytic cycle (Figure 1) by a rapid (9 × 10^6 M^-1 s^-1) two-electron oxidation of the ferric ground-state HRP to Compound I which is a porphyrin-p-cation radical.
Entered by Uri M
ID 104240