Rate of activation of phosphodiesterase (PDE) catalytic subunits per photoisomerized rhodopsin

Value 80 sec^-1 Range: maximum 90 sec^-1
Organism vertebrates
Reference Leskov, I. B., V. A. Klenchin, J. W. Handy, G. G. Whitlock, V. I. Govardovskii, M. D. Bownds, T. D. Lamb, E. N. Pugh, Jr., and V. Y. Arshavsky. 2000. The gain of rod phototransduction: reconciliation of biochemical and electrophysiological measurements. Neuron. 27: 525– 537 p.525 right column bottom paragraphPubMed ID11055435
Primary Source Dumke CL, Arshavsky VY, Calvert PD, Bownds MD, Pugh EN Jr. Rod outer segment structure influences the apparent kinetic parameters of cyclic GMP phosphodiesterase. J Gen Physiol. 1994 Jun103(6):1071-98. & Melia TJ Jr, Cowan CW, Angleson JK, Wensel TG. A comparison of the efficiency of G protein activation by ligand-free and light-activated forms of rhodopsin. Biophys J. 1997 Dec73(6):3182-91.PubMed ID7931138, 9414230
Comments "Measurements of ?E are found to average 80sec^-1 with a maximum of 90sec^-1 (Pugh and Lamb, 1993, Table IV, after correction for the revised ratio of 1 PDE to 270 rhodopsins in amphibian rods from Dumke et al., 1994 Melia et al., 1997).
Entered by Uri M
ID 111005