| Value |
20
min
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Sezonov G, Joseleau-Petit D, D'Ari R. Escherichia coli physiology in Luria-Bertani broth. J Bacteriol. 2007 Dec189(23):8746-9 p.8746 right column 4th paragraphPubMed ID17905994
|
| Method |
P.8746 left column top paragraph: "The recipe for Luria-Bertani broth is as follows: Combine 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, and 1 liter of distilled water adjust the pH to 7.0 with 1 N NaOH and autoclave the mixture for 25 min at 120°C. The tryptone used is a pancreatic digest of casein from cow's milk, and the yeast extract used is an autodigest of Saccharomyces cerevisiae." P.8746 right column 3rd paragraph: "The widely used wild-type E. coli K-12 strain MG1655 (ref 1), which has been sequenced and annotated (refs 2, 10), was chosen for this study. An overnight culture in Luria-Bertani broth was diluted 5,000-fold in 250 ml fresh medium in a 1-liter Erlenmeyer flask and cultivated in a shaking water bath (180 rpm) at 37°C. The OD600 and number of cells per milliliter were monitored." |
| Comments |
p.8746, right column 4th paragraph: "The doubling time during steady-state growth in Luria-Bertani broth was 20 min (Fig. 1A)." P.8747 left column 3rd paragraph: "A culture of MG1655 was grown for 24 h in Luria-Bertani broth (final OD600 of 6.49)." P.8747 caption to fig.1: "An overnight culture was diluted 5,000-fold in Luria-Bertani broth and cultivated at 37°C with vigorous aeration." For minimal generation time of 18 min in LB broth see BNID 104331 |
| Entered by |
Uri M |
| ID |
103514 |