Escherichia coli physiology in Luria-Bertani broth

J Bacteriol. 2007 Dec;189(23):8746-9. doi: 10.1128/JB.01368-07. Epub 2007 Sep 28.

Abstract

Luria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD(600)) of 7. Surprisingly, however, steady-state growth ceases at an OD(600) of 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilizable carbon source. The carbon sources for E. coli in Luria-Bertani broth are catabolizable amino acids, not sugars.

MeSH terms

  • Amino Acids / chemistry
  • Amino Acids / metabolism
  • Carbohydrates / chemistry
  • Carbon / metabolism
  • Cell Proliferation / drug effects
  • Culture Media / chemistry*
  • Culture Media / pharmacology*
  • Escherichia coli / drug effects*
  • Escherichia coli / physiology*
  • Fermentation

Substances

  • Amino Acids
  • Carbohydrates
  • Culture Media
  • Carbon