| Value |
<=100
uM
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Sezonov G, Joseleau-Petit D, D'Ari R. Escherichia coli physiology in Luria-Bertani broth. J Bacteriol. 2007 Dec189(23):8746-9PubMed ID17905994
|
| Method |
P.8746 right column 3rd paragraph: "The widely used wild-type E. coli K-12 strain MG1655 (ref 1), which has been sequenced and annotated (refs 2, 10), was chosen for this study. An overnight culture in Luria-Bertani broth was diluted 5,000-fold in 250 ml fresh medium in a 1-liter Erlenmeyer flask and cultivated in a shaking water bath (180 rpm) at 37°C. The OD600 and number of cells per milliliter were monitored." P.8747 right column 2nd paragraph: "...[investigators] set up a bioassay for fermentable sugars by using a hemA derivative of MG1655. This mutant is unable to synthesize δ-aminolevulinic acid. In the absence of exogenous δ-aminolevulinate, it cannot synthesize heme and thus cannot produce cytochromes, making it unable to carry out respiration. The mutant therefore absolutely requires a fermentable sugar in order to grow in the absence of δ-aminolevulinate." |
| Comments |
Fermentable sugar equivalents=free sugars, sugar phosphates, oligosaccharides, nucleotides, etc. |
| Entered by |
Uri M |
| ID |
103510 |