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Reduction in number of endocytic sites on cell surface in clathrin mutants compared to wild type

Range	50-75 Percent
Organism	Budding yeast Saccharomyces cerevisiae
Reference	Kaksonen M, Toret CP, Drubin DG. A modular design for the clathrin- and actin-mediated endocytosis machinery. Cell. 2005 Oct 21123(2):305-20.Click here to readPubMed ID16239147
Method	TIRF microscopy and epifluorescence. Approximately spherical single and large budded cells with unpolarized patches were scored for each strain. Surface area of individual cells was estimated as an average of sphere surface areas calculated from four diameters (at 0°, 45°, 90°, and 135°) measured from maximum intensity projections.
Entered by	Uri M
ID	103218