Lifetime of patch formed by Rvs161-GFP in endocytic pathway

Value 12.1 sec Range: ±3.3 sec
Organism Budding yeast Saccharomyces cerevisiae
Reference Kaksonen M, Toret CP, Drubin DG. A modular design for the clathrin- and actin-mediated endocytosis machinery. Cell. 2005 Oct 21123(2):305-20.Click here to readPubMed ID16239147
Method TIRF microscopy and epifluorescence. Researchers quantitatively analyzed the spatial and temporal localization of the C-terminal GFP fusions of each protein expressed from its endogenous locus
Comments Rvs161-GFP and Rvs167-GFP also appeared at patches very transiently (12.1 ± 3.3 and 10.2 ± 1.4 s respectively Figure 3A). These proteins were initially immotile, then moved and immediately dissipated from the patch (Figures 3B and 3C). The patch centroids moved only about 100 nm, and the movement took place in less than 0.5 s
Entered by Uri M
ID 103221