| Value |
1
hour
|
| Organism |
Bacteriophage T5 |
| Reference |
Mangenot S, Hochrein M, Rädler J, Letellier L. Real-time imaging of DNA ejection from single phage particles. Curr Biol. 2005 Mar 815(5):430-5PubMed ID15753037
|
| Method |
fluorescence microscopy study investigating in real time the dynamics of DNA ejection from single T5 phages adsorbed onto a microfluidic cell. The ejected DNA was fluorescently stained, and its length was measured at different stages of the ejection after being stretched in a hydrodynamic flow. Use was made of a microfluidic device that combines the advantages of allowing phage particles to be adsorbed onto a surface and restricting the DNA to a confined volume, thus allowing its observation in focus. |
| Comments |
DNA release is not an all-or-none process but occurs in a stepwise fashion and at a rate reaching 75,000 bp/sec. This stepwise fashion is apparently due to nicks in the DNA |
| Entered by |
Uri M |
| ID |
103137 |