Duration of ejection of DNA into host cell

Value 10 minutes
Organism Bacteriophage T7
Reference Garcia HG, Grayson P, Han L, Inamdar M, Kondev J, Nelson PC, Phillips R, Widom J, Wiggins PA. Biological consequences of tightly bent DNA: the other life of a macromolecular celebrity. Biopolymers. 2007 Feb 585(2):115-30.PubMed ID17103419
Primary Source Kemp P, Gupta M, Molineux IJ. Bacteriophage T7 DNA ejection into cells is initiated by an enzyme-like mechanism. Mol Microbiol. 2004 Aug53(4):1251-65.PubMed ID15306026
Comments At 30°C about 10 min, or one-third of the complete T7 life cycle, are normally necessary for complete internalization of the genome. This slow, tripartite mode of genome entry is part of the mechanism by which T7 avoids restriction by type I restriction enzymes. By requiring transcription by E. coli RNA pol to internalize the early region of the phage genome, expression of the antirestriction activity of gene 0.3 is ensured
Entered by Uri M
ID 103132