Transcription rate of 90% of the genes

Range 2.33 and 29.7 mRNA molecules/hour
Organism Budding yeast Saccharomyces cerevisiae
Reference Pelechano V, Chávez S, Pérez-Ortín JE. A complete set of nascent transcription rates for yeast genes. PLoS One. 2010 Nov 16 5(11):e15442. p.5 left column 2nd paragraphPubMed ID21103382
Method Researchers developed genomic variants of the well-known run-on technique to evaluate the nascent TR (transcription rate) for most genes. In this technique (GRO, Genomic Run-on), elongating RNA pol molecules, that conserve the RNA, are forced to incorporate radioactive UTP for a short length.
Comments If researchers assume that the 4670 genes for which they have data are representative of the 5796 non-dubious ORFs, the total transcription for RNA pol II in a yeast cell growing in standard conditions is about 60200 mRNAs/h.
Entered by Uri M
ID 106767