Excited GFP lifetime in cell cytoplasm
| Value | 2.6 nanosecond |
|---|---|
| Organism | Chinese hamster ovary (CHO) |
| Reference | Swaminathan R, Hoang CP, Verkman AS. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophys J. 1997 Apr72(4):1900-7.PubMed ID9083693 |
| Method | GFP mutant S65T was purified from recombinant bacteria for solution studies, and expressed in CHO cell cytoplasm. |
| Entered by | Uri M |
| ID | 102559 |