Time for recovery of half of the GFP after photobleaching in cell cytoplasm

Value 83 ms Range: ±6 ms
Organism Chinese hamster ovary (CHO)
Reference Swaminathan R, Hoang CP, Verkman AS. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophys J. 1997 Apr72(4):1900-7.PubMed ID9083693
Method GFP mutant S65T was purified from recombinant bacteria for solution studies, and expressed in CHO cell cytoplasm.
Comments Photobleaching recovery of GFP in cytoplasm was 82±2% complete. Under the same bleach conditions, recovery half time (t1/2) for fluorescein was threefold faster than that for GFP.
Entered by Uri M
ID 102557