Range: ±0.3 unitless
||Swaminathan R, Hoang CP, Verkman AS. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophys J. 1997 Apr72(4):1900-7.PubMed ID9083693
||To determine the relative viscosity of cytoplasm compared to water (same viscosity as PBS), t1/2 for the irreversible recovery process (83 ± 6 ms) in cytoplasm was compared to t1/2 measured for diffusion of purified GFP-S65T in solutions of known viscosity
||GFP mutant S65T was purified from recombinant bacteria for solution studies, and expressed in CHO cell cytoplasm.