Concentration of dCTP in a 42fL yeast cell, measured from an asyncrhonous population
| Value | 18 µM |
|---|---|
| Organism | Budding yeast Saccharomyces cerevisiae |
| Reference | Koç A, Wheeler LJ, Mathews CK, Merrill GF. Hydroxyurea arrests DNA replication by a mechanism that preserves basal dNTP pools. J Biol Chem. 2004 Jan 2 279(1):223-30 DOI: 10.1074/jbc.M303952200 p.225 left column top paragraphPubMed ID14573610 |
| Method | DNA polymerase-based enzymatic assay, HPLC |
| Comments | P.225 left column top paragraph: "Assuming a cell volume of 42 fl (ref 34 BNID 100427), the concentrations of dTTP, dATP, dCTP, and dGTP were 70, 44, 18, and 15 μm, respectively." Note: This value is measured from asynchronous cells, dNTP levels increase several fold during S-phase |
| Entered by | Paul Jorgensen |
| ID | 101415 |