Concentration of dTTP in a 42fL yeast cell, cells measured asychronously

Value 70 µM
Organism Budding yeast Saccharomyces cerevisiae
Reference Koç A, Wheeler LJ, Mathews CK, Merrill GF. Hydroxyurea arrests DNA replication by a mechanism that preserves basal dNTP pools. J Biol Chem. 2004 Jan 2 279(1):223-30 DOI: 10.1074/jbc.M303952200 p.225 left column top paragraphPubMed ID14573610
Method DNA polymerase-based enzymatic assay, HPLC
Comments P.225 left column top paragraph: "Assuming a cell volume of 42 fl (ref 34 BNID 100427), the concentrations of dTTP, dATP, dCTP, and dGTP were 70, 44, 18, and 15 μm, respectively." Note that this value is for asynchronous cultures, dNTP levels increase several fold at S-phase
Entered by Paul Jorgensen
ID 101413