Concentration of rATP in a yeast cell

Value 2 mM
Organism Budding yeast Saccharomyces cerevisiae
Reference Koç A, Wheeler LJ, Mathews CK, Merrill GF. Hydroxyurea arrests DNA replication by a mechanism that preserves basal dNTP pools. J Biol Chem. 2004 Jan 2 279(1):223-30 DOI: 10.1074/jbc.M303952200 p.229 right column bottom paragraphPubMed ID14573610
Primary Source [40] Exinger F, Lacroute F. 6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae. Curr Genet. 1992 Jul22(1):9-11 [41] Larsson C et al., Glycolytic flux is conditionally correlated with ATP concentration in Saccharomyces cerevisiae: a chemostat study under carbon- or nitrogen-limiting conditions. J Bacteriol. 1997 Dec179(23):7243-50.PubMed ID1611672, 9393686
Method Calculated from published data
Comments P.229 right column bottom paragraph: "The mechanism for replication arrest when RNR [ribonucleotide reductase] is inhibited remains unknown. If RNR inhibition resulted in significant expansion of the rNTP pools, it is possible that an increased rNTP/dNTP ratio might result in misincorporation of ribonucleotides into DNA and thereby trigger a replication arrest. However, the rNTP pools in yeast, calculated from published data to be 0.34 mM rUTP, 2 mM rATP, 0.26 mM rCTP, and 0.34 mM rGTP (primary sources), are 5–90-fold greater than the corresponding dNTP pools (Fig. 1B) (refs 15, 18) and are therefore unlikely to be affected by the presence or absence of a relatively small flux of rNDPs through RNR."
Entered by Paul Jorgensen
ID 101418