Cell concentration for culture with OD600 of 0.1

Value 1e+8 cell/ml
Organism Bacteria Escherichia coli
Reference Short protocols in molecular biology, Fred Ausubel et al., 5th ed. Vol. 1 pp. 1-6
Comments The optical density of yeast and bacterial cultures is due to light scattering, not absorption. Therefore it is sensitive to the geometry of the detector relative to the cuvette, as well as the size and shape of the cells. One must calibrate each spectrophotometer for each strain. (comment contributed by user mrose). Note that cell concentration of Luria-Bertani grown strain with OD600 of 0.1 is 2×10^7 cells/ml, smaller by a factor of 5 (see BNID 103625). See BNID 104831
Entered by Ron Milo - Admin
ID 100985