Cell concentration for culture at stationary phase with OD600 of 1 at 20°C

Value 1.1e+7 Cells/ml
Organism Budding yeast Saccharomyces cerevisiae
Reference Margesin R. Effect of temperature on growth parameters of psychrophilic bacteria and yeasts. Extremophiles. 2009 Mar13(2):257-62 p.261 fig.2 right bottom graphPubMed ID19057843
Method Culture turbidity (optical density) was measured spectrophotometrically a 600 nm (OD600). Numbers of viable cells were determined by the plate count method on the bacterial or yeast medium. Value extracted manually from fig. 2
Comments The optical density of yeast and bacterial cultures is due to light scattering, not absorption. Therefore it is sensitive to the geometry of the detector relative to the cuvette, as well as the size and shape of the cells. You really have to calibrate each spectrophotometer for each strain.
Entered by Uri M
ID 106300