Cell concentration for culture at stationary phase with OD600 of 1 at 10°C

Value 9e+6 Cells/ml
Organism Budding yeast Saccharomyces cerevisiae
Reference Margesin R. Effect of temperature on growth parameters of psychrophilic bacteria and yeasts. Extremophiles. 2009 Mar13(2):257-62 p.261 fig.2 right bottom graphPubMed ID19057843
Method P.258 right column 2nd paragraph: "Culture turbidity (optical density) was measured spectrophotometrically a 600 nm (OD600). Numbers of viable cells were determined by the plate count method on the bacterial or yeast medium as described above, solidified with agar (15 g/l). Colony-forming units (cfu) were counted after 2 days at 25°C (Pedobacter heparinus, Saccharomyces cerevisiae), 3 days at 20°C (Pedobacter piscium, P.heparinus, Leucosporidiella creatinivora), or after 5 days at 15°C (Rhodotorula glacialis). Only plates containing 30–300 colonies were used for statistically valid enumeration (Koch 1994)." Value extracted visually from fig. 2
Comments The optical density of yeast and bacterial cultures is due to light scattering, not absorption. Therefore it is sensitive to the geometry of the detector relative to the cuvette, as well as the size and shape of the cells. You really have to calibrate each spectrophotometer for each strain.
Entered by Uri M
ID 106299