Cell concentration for culture with OD600 of 0.1
| Value | 1e+8 cell/ml |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Short protocols in molecular biology, Fred Ausubel et al., 5th ed. Vol. 1 pp. 1-6 |
| Comments | The optical density of yeast and bacterial cultures is due to light scattering, not absorption. Therefore it is sensitive to the geometry of the detector relative to the cuvette, as well as the size and shape of the cells. One must calibrate each spectrophotometer for each strain. (comment contributed by user mrose). Note that cell concentration of Luria-Bertani grown strain with OD600 of 0.1 is 2×10^7 cells/ml, smaller by a factor of 5 (see BNID 103625). See BNID 104831 |
| Entered by | Ron Milo - Admin |
| ID | 100985 |