| Value |
187000
Ribosomes/cell
Range: ±56000 Table - link Ribosomes/cell
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
von der Haar T. A quantitative estimation of the global translational activity in logarithmically growing yeast cells. BMC Syst Biol. 2008 Oct 162:87 p6/14 table 1PubMed ID18925958
|
| Primary Source |
[30] Warner JR. The economics of ribosome biosynthesis in yeast, Trends Biochem Sci. 1999 Nov24(11):437-40 and see refs beneath tablePubMed ID10542411
|
| Method |
(For primary source [30], value of 200000 ribosomes/cell) Based on comparison of the size of the genome (1.4×10^7 bp) with the RNA in a ribosome (5469 nucleotides), and using ratio of DNA to rRNA |
| Comments |
(For von der Haar ref above p.5 right column bottom paragraph:) "The RP [ribosomal protein] abundance distribution in figure 2 shows a mean
abundance of 227,000 RPs per cell. Independent estimates for the cellular ribosome content have been generated by analysing the abundance of ribosomal RNA (rRNA) species in several studies [25-30], with reported values ranging from 150,000 to 350,000 copies of ribosomal RNA per cell for fast-growing haploid yeast strains. All of these studies were relying on relatively inaccurate
estimates for the molecular weights of rRNAs derived
from gel electrophoreses, and much of the variation in
reported rRNA abundances derives from the fact that different
estimates for this parameter were used. However,
these studies also report the raw data for total cellular
RNA content and the proportion of RNA that is rRNA, and
rRNA abundances are therefore here re-calculated from
these raw data based on the exact rRNA molecular weights
from now available sequence information (table 1). The
resulting estimate of 187,000 ± 56,000 rRNA copies per
yeast cell can be usefully compared to the distribution of
RP abundances in figure 2." See note under table. 300,000 ribosomes per generation given by Phizicky et al., 2010 PMID 20810645 p.1832 right column top paragraph (BNID 113860) |
| Entered by |
Ron Milo - Admin |
| ID |
100267 |