Translation rate of beta-galactosidase in E. coli

Value 14.5 aa/s Range: 13-16 aa/s
Organism Bacteria Escherichia coli
Reference Dalbow DG, Young R. Synthesis time of beta-galactosidase in Escherichia coli B/r as a function of growth rate. Biochem J. 1975 Jul150(1):13-20. abstract, p.15 table 2 and p.19 right column 3rd paragraphPubMed ID1106403
Method Analysis of the kinetics of ß-galactosidase accumulation after induction: "Cultures, on reaching a turbidity (E460) of 0.1, were treated with cyclic AMP (Sigma Chemical Co., St. Louis, Mo., U.S.A.) to a final concentration of 0.01 M, to decrease catabolite repression. Then 5 min later synthesis of ß-galactosidase was induced by the addition of the inducer isopropyl-ß-D-thiogalactopyranoside (Sigma), to a final concentration of 0.01 M." "ß-Galactosidase activity was assayed as described by Dalbow et al., 1975 (PMID 173297) except that the incubation at 30°C was allowed to continued for 24h."
Comments "In summary, [investigators’] results indicate that the peptide-chain-elongation rate is about 16 amino acids/s for fast-growing cells but is decreased to about 13 amino acids/s for slowly growing cells. These values may actually slightly underestimate the true peptide-chain-elongation rates, since they are based on the proposition that the synthesis time equals the translation time for ß-galactosidase." See BNID 100059,105067
Entered by Paul Jorgensen
ID 100233