DNA replication speed in vivo

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Range ~600 bp/sec
Organism Bacteria Escherichia coli
Reference Reyes-Lamothe R, Possoz C, Danilova O, Sherratt DJ. Independent positioning and action of Escherichia coli replisomes in live cells. Cell. 2008 Apr 4 133(1):90-102. doi: 10.1016/j.cell.2008.01.044. p.93 right column 2nd paragraphPubMed ID18394992
Method "Fluorescent C-terminal fusion-protein derivatives of E. coli replisome components, expressed from their endogenous chromosomal promoters, were constructed and their phenotypes assessed by growth, flow cytometry, and microscopy."
Comments "Five minutes of replication corresponds to ~180kb DNA replicated for each fork, ample time to give the observed spatial separation of two independent forks." From this one can infer a value of 600bp/s for the replication rate in vivo (180,000bp/300s).
Entered by Uri M
ID 109251