Length of S phase (time to replicate chromosome)

Range ~55 to 65 minutes
Organism Bacteria Escherichia coli
Reference Reyes-Lamothe R, Possoz C, Danilova O, Sherratt DJ. Independent positioning and action of Escherichia coli replisomes in live cells. Cell. 2008 Apr 4 133(1):90-102. doi: 10.1016/j.cell.2008.01.044. p.93 right column top paragraphPubMed ID18394992
Method "Fluorescent C-terminal fusion-protein derivatives of E. coli replisome components, expressed from their endogenous chromosomal promoters, were constructed and their phenotypes assessed by growth, flow cytometry, and microscopy."
Comments "Foci appear either shortly after birth or shortly before birth in a mother cell and are present for 65 min on average. Assuming the times of appearance and disappearance of foci mark replication initiation and termination, respectively, then this value for S phase, determined for timelapse cells growing on slides, is slightly longer than [researchers'] previous estimate of 55 min for cells growing in the same liquid medium (Wang et al., 2005 BNID 109249)."
Entered by Uri M
ID 109252