||Budding yeast Saccharomyces cerevisiae
||Joseph SB, Hall DW. Spontaneous mutations in diploid Saccharomyces cerevisiae: more beneficial than expected. Genetics. 2004 Dec168(4):1817-25 p.1820 right column top paragraphPubMed ID15611159
||P.1818 left column 4th paragraph: "MA [mutation-accumulation] line establishment and propagation: One hundred fifty-one MA lines were established from the ancestor. Each MA line was grown on YPD solid medium (1% yeast extract, 2% peptone, 2% dextrose, and 2% agar) at 30° and passaged by single-cell transfer." P.1818 right column 2nd paragraph: "[Researchers] estimated the average number of generations assuming exponential growth from counts of the number of cells per colony. The number of cells per colony was estimated approximately every seven transfers by choosing a single colony from each of 10 petri dishes. [They] then suspended the colony in 1 ml of water and estimated cell density using a hemacytometer. (Reichert Bright Line, 0.1 mm depth)."
||P.1820 right column top paragraph: "For forty-eight of the 50 passages, average colony size was estimated to be 1.4×10^6 cells, which represents ~20.4 generations between passages, or one cell division every 141 min." YPD Medium is a blend of peptone, yeast extract, and dextrose in optimal proportions for growing most Saccharomyces cerevisiae strains.
||Ron Milo - Admin