Number of RNA polymerase and general transcription factor protein molecules per haploid cell

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Borggrefe T, Davis R, Bareket-Samish A, Kornberg RD. Quantitation of the RNA polymerase II transcription machinery in yeast. J Biol Chem. 2001 Dec 14 276(50):47150-3 p.47152 table IIPubMed ID11591727
Method Tandem Affinity Purification (TAP) tags and dot blot analysis have been used to measure the amounts of RNA polymerase II transcription proteins in crude yeast extracts.
Comments "The results (Table II) showed the greatest abundance of RNA polymerase II, in keeping with its involvement in both the initiation and elongation of transcription. The level of TFIIF was nearly the same, as expected from its tight association with RNA polymerase II and from its likely role in both transcription initiation and elongation as well (ref 16)." Range of the 6 TF copies in table is 6,000-24,000 proteins/cell. Note-(1) Frank A. Robey, 'Use of capillary electrophoresis for binding studies' in James P Landers, Handbook of capillary electrophoresis, 1996, pp 598 writes: " is believed that there are only 10^3-10^4 molecules of transcription factors per cell nucleus,...". (2) Bolouri et al, 2003 PMID 12883007 give value of 300-10000 TF proteins/cell in sea urchin.
Entered by Uri M
ID 104515