Table - link % of carbon recovered
||Bacteria Bacillus subtilis
||Dauner M, Storni T, Sauer U (2001) Bacillus subtilis metabolism and energetics in carbon-limited and excess-carbon chemostat culture. J Bacteriol 183: 7308–7317 p.7311 table 1PubMed ID11717290
||See ref beneath table
||p.7309 left column 2nd paragraph:"The primary focus of this work was to quantify the magnitude
of energy dissipation via overflow metabolism, metabolic
shifts, and futile enzyme cycling in C-limited and excess-C
Bacillus subtilis chemostat cultures, by using isotopomer-balancing
(ref 9) and [U-13C6]glucose-labeling experiments (ref 55).
Thus, for the first time [investigators] report here intracellular carbon flux
distributions in N- and P-limited B. subtilis."
||p.7311 left column bottom paragraph:"Formation of low-molecular-weight by-products (excluding riboflavin) by so-called overflow metabolism was low in C-limited culture (ref 10 beneath table), moderate in N-limited culture, and extensive in P-limited culture (Table 1), as was described for Bacillus spp. (refs 28, 35) and E. coli (ref 31). The primary by-product was acetate, but diacyl and acetoin formation was also significant. The specific production rate of acetate was strongly influenced by the growth rate and was highest under P limitation (Fig. 3). Not generally considered classical products of overflow metabolism, extracellular protein and cell wall components contributed 1 to 4% of the carbon balance (data not shown). Recombinant riboflavin production contributed very little to the carbon balance and showed no pronounced response to the different environmental conditions (Table 1)."